Structural Fat Grafting | Fat Harvesting, Purification, and Coleman Technique

The harvested lipoaspirate contains blood, tumescent fluid, ruptured lipid droplets, and intact adipocytes. Centrifugation (at 3000 RPM for 3 minutes) or sterile gravity filtration isolates the dense, viable adipocyte layer and adipose-derived regenerative cells. The purified graft is then transferred into 1 mL micro-syringes and layered meticulously through tiny 1 mm entry ports into multiple tissue depths (subcutaneous, intramuscular, and pre-periosteal).

Macrofat consists of large adipose parcels ideal for buttock or deep breast augmentation. Microfat particles are gently calibrated through 1 mm sizing ports for the cheeks, temples, and jawline. Nanofat is mechanically emulsified and filtered through specialized mesh, destroying mature fat cells while concentrating regenerative mesenchymal stem cells, vascular endothelial growth factors, and stromal vascular fraction (SVF) for microneedling and treating superficial fine lines, dark circles, and scars.